A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues
Journal of Biological Chemistry, 1957 · paper · Biomedicine
Describes a simplified method for extracting and purifying total lipids from animal tissues, using chloroform-methanol extraction followed by washing with a small volume of water. The authors report it is simpler, scalable, and causes less lipid loss than the original method.
Checked: PDF from diverdi.colostate.edu, fetched 5 Oct 2026. Page numbers are those of that PDF.
0 findings
None that passed a second check.
2 minor (typos and the like; most checked by the judge only)
Wording "This difference, which amounts to <0.5 per cent of total tissue lipides": for the muscle experiment described as "identical" to the 40 gm. liver run, 3.5 mg. is about 0.6 per cent of the total lipides implied by 0.743 mg. per ml.
- p. 5 Degree of Completeness of Extraction, muscle experiment
This difference, which amounts to <0.5 per cent of total tissue lipides,
- p. 5 Same paragraph
contained 0.743 mg. of lipides per ml., while 24.3 ml. of extract with a total
- p. 5 Liver experiment, which the muscle experiment is said to be identical to
40 gm. of tissue were
- p. 5 Degree of Completeness of Extraction, muscle experiment
amounts to <0.5 per cent of total tissue lipides
Only the authors can say what is right here.
Judge's note: Viewed p 5 and Table II on p 7. The paragraph's other figures check (24.3 x 0.743 = 18.1; 21.6 - 18.1 = 3.5). The liver run used 40 gm. and 760 ml. of solvent, i.e. about 800 ml. of first extract at the standard 20-fold dilution (2.46 x 800 = 1968 mg, 49 mg/g, matching Table II liver). The muscle run is called 'an identical experiment'; at 0.743 mg/ml x 800 ml the total is about 594 mg and 3.5 mg is 0.59%. Table II muscle (0.26 mg/g = 1.8%, i.e. about 14.4 mg/g) gives the same total for 40 gm. The muscle sample weight is not actually stated, so more than about 47 gm. of muscle would make '<0.5' true; only the authors can say. The conclusion (difference not significant) is unaffected.
Second check: another model agreed
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Wording "lipides other than strandin lost in the course of the first washing amounted to no more than 0.6 per cent of the tissue lipides" for gray matter, but Table II lists 0.65 per cent for the fourth gray-matter experiment.
- p. 6 Text below Table I
the first washing amounted to no more than 0.6 per cent of the tissue lip-
- p. 7 Table II, Gray matter, 4th row, 1st upper phase, per cent tissue lipides
0.65
- p. 6 Text below Table I
amounted to no more than 0.6 per cent of the tissue lip-
- p. 13 Summary 2
about 0.6 per cent in
Judge's note: Viewed p 6 and Table II on p 7. Gray matter, 1st upper phase, per cent tissue lipides: 0.56, 0.57, 0.31, 0.65 (unstarred, so lipides other than strandin). The 0.65 is consistent with its own row (0.44 mg/g; 2nd phase 0.9 mg/g at 1.34%). The stated ceiling is exceeded by 0.05 percentage points; this is loose rounding of a bound, not a wrong result. The Summary's 'about 0.6 per cent' is fine.
Second check: another model agreed