Single Lgr5 Stem Cells Build Crypt-Villus Structures In Vitro without a Mesenchymal Niche
Nature, 2009 · paper · Biomedicine
Establishes long-term culture conditions in which mouse intestinal crypts, or single Lgr5-positive stem cells, grow into crypt–villus organoids containing all differentiated cell types. The authors conclude these structures self-organize without a non-epithelial niche.
1 finding
Reference or label Fig. 3 legend: "Error bars in c and d indicate s.e.m." Panel d is a micrograph with no error bars; the panels with error bars are a and c.
- p. 3 Figure 3 legend
Error bars in c and d indicate s.e.m.
Judge's note: The p3 image shows panel a (colony-forming efficiency bar chart) with an error bar on the GFPhi bar and panel c with error bars. Panel d is a brightfield photograph only.
Second check: another model agreed
Report a problem with this finding
10 minor (typos and the like; most checked by the judge only)
Reference or label "the Cre-activatable Rosa26–YFP reporter19,20 mouse" cites ref. 20 (Soriano, "Generalized lacZ expression with the ROSA26 Cre reporter strain"), while the Rosa26–LacZ reporter mentioned in the preceding sentences has no citation.
- p. 1 Main text, right column
the Cre-activatable Rosa26–YFP reporter19,20 mouse
- p. 4 Reference 20
Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain.
Judge's note: Page 1 shows the 19,20 superscripts attached to Rosa26–YFP and no citation on 'Rosa26–LacZ reporter'. Page 4 shows ref. 20 is the lacZ reporter paper. Citing Soriano for the ROSA26 reporter system in general is defensible, so this is a placement point rather than a wrong citation.
Second check: another model agreed
Report a problem with this finding
Reference or label Fig. 4 legend: "alkaline phosphatase (e, green, enterocytes)". Panel e is a brightfield paraffin section with a dark apical stain, and no green is visible.
- p. 3 Figure 4 legend, panel e
alkaline phosphatase (e, green, enterocytes)
Only the authors can say what is right here.
Judge's note: In the p3 page image, panel e shows pale pink cells with a dark apical band. Neighbouring brightfield panels f–h reproduce their stated colours, but panel e shows no green. Only the authors can say what colour was intended.
Second check: another model agreed
Report a problem with this finding
Wording Fig. 3a legend: "Colony-forming efficiency was calculated from 100 single sorted GFPhi cells", but the panel also plots Lgr5–GFPlow, and the text says "In each of four individual experiments, we identified and followed 100 single cells."
- p. 3 Figure 3 legend, panel a
Colony-forming efficiency was calculated from 100 single sorted GFPhi cells.
- p. 2 Main text, right column
In each of four individual experiments, we identified and followed 100 single cells.
Only the authors can say what is right here.
Judge's note: Panel a on p3 shows GFPlow and GFPhi bars with an error bar. The legend gives no denominator for GFPlow and does not state the number of experiments. '100 cells' may mean per experiment, so this is an incompleteness for the authors to clarify, not a contradiction.
Second check: another model agreed
Report a problem with this finding
Wording Methods: "an inverted microscope (Nikon DM-IL)". DM IL appears to be a Leica model, and the same sentence lists Leica instruments.
- p. 5 Methods, Image analysis
an inverted microscope (Nikon DM-IL)
Only the authors can say what is right here.
Judge's note: Rests on outside knowledge: that the DM IL inverted microscope is made by Leica, not Nikon. The page 5 image shows 'Nikon DM-IL'.
Second check: another model agreed
Report a problem with this finding
Typo Abstract misspelling: "while simultanously generating villus-like epithelial domains" should read 'simultaneously'.
- p. 1 Abstract
while simultanously generating villus-like epithelial
- p. 1 Abstract
simultanously generating villus-like epithelial
Judge's note: Verified in the page 1 image of the abstract; the word is printed 'simultanously'.
Second check: none (judge only)
Report a problem with this finding
Typo Misspelling: "reminiscent of the shedding of apopotic cells at villus tips in vivo" should read 'apoptotic'.
- p. 1 Main text, right column
reminiscent of the shedding of apopotic cells at villus tips
- p. 1 Main text, right column
shedding of apopotic cells at villus tips
- p. 1 Results paragraph 3
shedding of apopotic cells at villus tips in vivo
Judge's note: Verified in the page 1 image, right column.
Second check: none (judge only)
Report a problem with this finding
Typo The allele is written "Lgr5–EGFP–Ires–CreERT2" in the Methods but "Lgr5–EGFP–ires–CreERT2" in the main text and Fig. 2 legend.
- p. 4 Methods Summary, Mice
Lgr5–EGFP–Ires–CreERT2
- p. 1 Main text
Lgr5–EGFP–ires–CreERT2
Judge's note: Text-layer search confirms 'ires' on p1 and p2 and 'Ires' in the Methods on p4; the p1 image shows 'ires'. The difference is cosmetic only.
Second check: none (judge only)
Report a problem with this finding
Typo Methods misspelling: "0.1 M sodiumm cacodylate" should read 'sodium cacodylate'.
- p. 5 Methods, Electron microscopic analysis
0.1 M sodiumm cacodylate
Judge's note: Verified in the page 5 image.
Second check: none (judge only)
Report a problem with this finding
Typo Methods: "examined with a Phillips CM10 microscope". The manufacturer is spelled Philips.
- p. 5 Methods, Electron microscopic analysis
Phillips CM10 microscope
Only the authors can say what is right here.
Judge's note: Rests on outside knowledge: that the CM10 TEM is made by Philips (one 'l'). The page 5 image shows 'Phillips'.
Second check: none (judge only)
Report a problem with this finding
Typo Stray full stop in "were centrifuged at 150–200g. for 3 min".
- p. 5 Methods, Crypt isolation
150–200g. for 3 min
Judge's note: Verified in the page 5 image: a period follows the italic g in mid-sentence.
Second check: none (judge only)