Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors

• p. 3 Results, High Telomerase Activity section

  They proliferated exponentially for as least 4 months (Figure 4B)

• p. 5 Figure 4B x-axis

  Days

• p. 3 Results

  proteins levels of OCT3/4

• p. 3 Telomerase

  for as least 4

Only the authors can say what is right here.

Judge's note: The page 3 crop shows the sentence citing Figure 4B. The page 6 image shows Figure 4B with a Days axis from 0 to 60 and data ending near day 58–60. So the cited panel shows about 2 months of growth, not 4. The cells may have been cultured for longer without being plotted, so this is a query for the authors. Other copyedit slips in this cluster are visible on the page images: 'proteins levels' (p3), 'iPS cells clones' (p3), 'This is great contrast' (p8). The slip 'as least' is handled under C005.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 3 Results, High Telomerase Activity section

  47.8 ± 6.6 (201B6) and 43.2 ± 11.5 (201B7) hours

• p. 6 Figure 4B

  Cell number (log10)

• p. 3 Results, High Telomerase Activity section

  46.9 ± 12.4 (clone 201B2), 47.8 ± 6.6 (201B6) and 43.2 ± 11.5 (201B7) hours

Only the authors can say what is right here.

Judge's note: Page 6 image: Figure 4B rises from 10^5 at day 0 to just over 10^11 at about day 58–60. That is about 6.3 log10 units, or about 20.9 doublings in about 1392 h, which gives about 66 h per doubling. At 47 h per doubling the cells would reach roughly 10^14. Neither the Results nor the Methods say how the doubling times were calculated (for example, per passage or over the steepest phase). This is a query for the authors, not a proven error.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 5 Figure 3B legend

  were introduced into human iPS cells or HDF by lipofection

• p. 3 Results

  in human iPS cells and EC cells (NTERA-2) but not in HDF

• p. 10 Experimental Procedures, Luciferase Assay

  introduced into human iPS cells or HDF with 50 ng of pRL-TK

Judge's note: Page 5 image: all three luciferase panels have an NTERA-2 bar. The legend and the Luciferase Assay methods (p10) name only iPS cells and HDF. The p3 text reports activity in 'EC cells (NTERA-2)'. This is an omission in the description.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 8 Discussion, first paragraph

  The four retroviruses are strongly silenced in human iPS cells

• p. 3 Results, Human iPS Cells Express hES Markers

  confirmed efficient silencing of all the four retroviruses

• p. 4 Figure 2C, OCT3/4 Tg panel

  OCT3/4 Tg

Judge's note: Page 4 image, Figure 2C: the OCT3/4 Tg panel shows clone 3 at about 0.25, with an error bar up to about 0.45. All other clones and transgenes are near zero. A drop of about 75% can reasonably be called silencing, so this is a minor point about the unqualified wording.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 4 Results, Directed Differentiation into Neural Cells

  maintained them under differentia-tion conditions for 2 weeks

• p. 7 Figure 6A legend

  after 18 days cultivation on PA6

• p. 4 Directed Differentiation into Neural Cells

  differentia-tion conditions for 2 weeks

Only the authors can say what is right here.

Judge's note: Page 4 crop: 'maintained them under differentiation conditions for 2 weeks ... (Figure 6A)'. Page 7 crop: the Fig 6A legend says '18 days cultivation on PA6'. The Methods (p10, In Vitro Differentiation) give no PA6 duration. The image may have been taken later than the main endpoint, but the paper does not say so.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 11 Experimental Procedures, DNA Microarray

  hiPS cells (clone 201B)

• p. 4 Figure 2D legend

  human iPS cells (clone 201B7)

• p. 11 Experimental Procedures, DNA Microarray

  Total RNA from HDF and hiPS cells (clone 201B) was labeled with Cy3.

• p. 11 Acknowledgments

  Dr. Peter. W. Andrews

Only the authors can say what is right here.

Judge's note: I checked the page images for p. 11 and p. 4. Every other mention in the text names a specific clone (201B2, 201B6, 201B7), and the microarray legend says 201B7. So the methods identifier is incomplete and probably means 201B7, but only the authors can confirm which clone was arrayed. The stray period in 'Dr. Peter. W. Andrews' in the Acknowledgments is visible too (cosmetic).

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 8 Discussion

  each clone had more than 20 retroviral integration sites in total

• p. 8 Discussion, right column, last paragraph

  each iPS clone contained three to six ret-roviral integrations for each factor. Thus, each clone had more than 20 retroviral integration sites in total

• p. 8 Discussion

  three to six ret-roviral integrations for each factor. Thus, each clone had more than 20 retroviral integration sites in total

• p. 8 Discussion

  three to six ret- roviral integrations for each factor. Thus, each clone had more than 20 retroviral integration sites

Only the authors can say what is right here.

Judge's note: The page 8 image confirms the wording. Four factors times 3 to 6 integrations gives totals from 12 to 24, so 'Thus ... more than 20' holds only if most factors are at the top of the range. Figure S3B, which would show the actual counts, is not in the files.

Second check: another model agreed

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 2 Generation of iPS cells

  The HDF de- rived from facial dermis

Judge's note: Page 2 image, left column under 'Generation of iPS Cells from Adult HDF': the sentence ends after 'female.' with no finite verb. This is a cosmetic grammar slip.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 3 Results, High Telomerase Activity section

  for as least 4

• p. 3 Results, High Telomerase Activity section

  for as least 4 months

Judge's note: The page 3 crop shows 'as least'. This is a cosmetic typo.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 5 Results, Neural differentiation

  member 3 (DAT)

Judge's note: Page 5 crop: the text runs straight from 'decarboxylase (AADC),' to 'member 3 (DAT)' within one line, so no line has been lost in extraction. The DAT abbreviation still identifies the gene, so this is cosmetic.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 5 Figure 3 title

  Analyses Promoter Regions of Development-Associated Genes

• p. 7 Heading

  not Cross contamination

Judge's note: The page 5 image shows the title as quoted. The heading-capitalisation point ('not Cross contamination', p7) is a trivial style matter and is not counted separately.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 10 Experimental Procedures, In Vitro Differentiation

  bone morphologenic protein

• p. 5 Results, Cardiac differentiation

  bone morphogenetic protein (BMP)

Judge's note: Both spellings are visible in the page images: 'morphologenic' in the methods on PDF page 10 and 'morphogenetic' in the Results on PDF page 5.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 8 Results, Generation of iPS Cells from Other Human Somatic Cells

  more than 600 hundred granulated colonies

• p. 8

  we obtained more than 600 hundred granulated colonies

Judge's note: The page 8 image (Generation of iPS Cells from Other Human Somatic Cells) shows '600 hundred'. This is a wording slip.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 8 Discussion, third paragraph

  cannot bind their tar-gets genes

Judge's note: Page 8 image, right column: 'their tar-gets genes'. This is a cosmetic grammar slip.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 8 Other somatic cells

  (Table S1 and S2)

Judge's note: Page 8 image confirms both forms. The difference is purely cosmetic. Mixing 'hiPS' and 'human iPS' is a style matter.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 9 Experimental Procedures, Cell Culture

  DMEF/F12/collangenase was removed

• p. 9 Cell Culture

  DMEF/F12/collangenase

• p. 11 ChIP

  share chromatin-DNA complex

• p. 10 In Vitro Differentiation

  poly (2-hydroxyrthyl methacrylate)

Judge's note: Each misspelling is visible in the crops of pages 9, 10 and 11. The previous sentence on page 9 names 'DMEM/F12 containing 1 mg/ml collagenase IV'. These are cosmetic typos.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 10 Experimental Procedures, In Vitro Differentiation

  (2-hydroxyrthyl methacrylate)

• p. 10 In Vitro Differentiation

  bone morphologenic

Judge's note: The page-10 image shows 'hydroxyrthyl', so this is a real misspelling. The 'morphologenic' misspelling that reviewer note 2 also mentions is covered separately in C017.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 10 Experimental Procedures, Retroviral Infection (continued)

  dissociated to small clamps by pipeting

• p. 10 Retroviral Infection

  small clamps by pipeting

• p. 10

  small clamps by pipeting up and down

Judge's note: The page-10 image shows 'clamps'. Elsewhere the paper says 'small clumps' (Results, and In Vitro Differentiation on the same page). 'pipeting' is a less common spelling but is not clearly an error.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 10 Experimental Procedures, Teratoma Formation

  tumors were dissected, weighted, and fixed

• p. 10 Teratoma Formation

  dissected, weighted, and fixed

Judge's note: The page-10 image shows 'weighted'. 'Weighed' is the intended word.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 10 Immunocytochemistry

  Nucleuses were

• p. 10 Western Blotting

  polyvinylidine

Judge's note: Both forms are visible in the page-10 image, in the Immunocytochemistry and Western Blotting sections.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 11 Experimental Procedures heading

  Chromatin immunoprecuipitation Assay

Judge's note: The page-11 image shows the misspelt heading. The body text directly below it spells 'Immunoprecipitation' correctly.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send

• p. 11 Experimental Procedures, ChIP

  sonicated to share chromatin-DNA complex

Judge's note: The page-11 image shows 'share'. Sonication shears chromatin, so the word is wrong.

Second check: none (judge only)

Report a problem with this finding

This finding is

wrong right unclear or overstated already corrected (erratum, later version)

I am an author of this paper Send